{"id":2463,"date":"2012-06-29T13:30:43","date_gmt":"2012-06-29T17:30:43","guid":{"rendered":"http:\/\/labrigger.com\/blog\/?p=2463"},"modified":"2012-06-29T20:47:02","modified_gmt":"2012-06-30T00:47:02","slug":"whole-embryo-imaging","status":"publish","type":"post","link":"http:\/\/labrigger.com\/blog\/2012\/06\/29\/whole-embryo-imaging\/","title":{"rendered":"Whole embryo imaging"},"content":{"rendered":"<p align=\"center\"><a href=\"http:\/\/www.nature.com\/nmeth\/journal\/v9\/n7\/full\/nmeth.2022.html\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2012\/06\/setup.png\" alt=\"\" title=\"Click for source.\" width=\"600\" height=\"565\" class=\"aligncenter size-full wp-image-2464\" srcset=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2012\/06\/setup.png 600w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2012\/06\/setup-300x282.png 300w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><\/a><\/p>\n<p>Drosophila embryos are a fraction of a square mm, and go from fertilization to hatching (as a larva) in about 22 hrs. So it&#8217;s possible to image individual embryos in their entirety with minute or sub-minute level temporal resolution (each 3D snapshot takes about 30-500 seconds, depending on method and resolution).<\/p>\n<p>A <a href=\"http:\/\/www.nature.com\/nmeth\/journal\/v9\/n7\/full\/nmeth.2064.html\">couple<\/a> of <a href=\"http:\/\/www.nature.com\/nmeth\/journal\/v9\/n7\/full\/nmeth.2062.html\">papers<\/a> exploring whole embryo imaging appear in the latest Nature Methods. The <a href=\"http:\/\/www.nature.com\/nmeth\/journal\/v9\/n7\/full\/nmeth.2022.html\">accompanying N&#038;V<\/a> does a nice job of summarizing the different approaches. Check out some of the movies. They&#8217;re spectacular.<\/p>\n<p align=\"center\"><a href=\"http:\/\/www.nature.com\/nmeth\/journal\/v9\/n7\/full\/nmeth.2062.html\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2012\/06\/mov.png\" alt=\"\" title=\"Click for source.\" width=\"600\" height=\"427\" class=\"aligncenter size-full wp-image-2465\" srcset=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2012\/06\/mov.png 600w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2012\/06\/mov-300x213.png 300w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p align=\"center\"><a href=\"http:\/\/www.nature.com\/nmeth\/journal\/v9\/n7\/full\/nmeth.2022.html\"><\/a><\/p>\n<p>Drosophila embryos are a fraction of a square mm, and go from fertilization to hatching (as a larva) in about 22 hrs. So it&#8217;s possible to image individual embryos in their&#8230;<\/p>\n<div class=\"read-more\"><a href=\"http:\/\/labrigger.com\/blog\/2012\/06\/29\/whole-embryo-imaging\/\">Read More<\/a><\/div><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[35,21,36,15],"class_list":["post-2463","post","type-post","status-publish","format-standard","hentry","category-hardware","tag-fluorescence","tag-imaging","tag-microscopy","tag-optics"],"_links":{"self":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/2463","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/comments?post=2463"}],"version-history":[{"count":5,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/2463\/revisions"}],"predecessor-version":[{"id":2470,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/2463\/revisions\/2470"}],"wp:attachment":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/media?parent=2463"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/categories?post=2463"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/tags?post=2463"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}