{"id":2505,"date":"2012-09-01T02:46:16","date_gmt":"2012-09-01T06:46:16","guid":{"rendered":"http:\/\/labrigger.com\/blog\/?p=2505"},"modified":"2012-08-29T12:44:33","modified_gmt":"2012-08-29T16:44:33","slug":"diy-superresolution-part-ii","status":"publish","type":"post","link":"http:\/\/labrigger.com\/blog\/2012\/09\/01\/diy-superresolution-part-ii\/","title":{"rendered":"DIY Superresolution, part II"},"content":{"rendered":"

\"\"<\/p>\n

This paper (York et al. 2012)<\/a> came out earlier this year, but I thought it’s worth highlighting here, give the subject matter.<\/p>\n

We present a hybrid technique, multifocal SIM (MSIM), that combines the resolution-doubling characteristics of SIM with the physical optical sectioning of confocal microscopy. MSIM uses sparse 2D excitation patterns generated with a DMD integrated into a conventional wide-field microscope and digital processing after acquisition (‘postprocessing’) to obtain optically sectioned images with ~145-nm lateral and ~400-nm axial resolution at 1-Hz frame rates. Relative to existing SIM, our implementation is easier to integrate onto existing microscopes and is considerably cheaper than commercial SIM.<\/p><\/blockquote>\n

For imaging, they used an sCMOS camera: the pco.edge<\/a> (I’ve demo’d one, they’re very cool). In the supplement, they offer some helpful tips on how to set up the camera to obtain high quality images. This section is a good example of how the authors carefully prepared the paper to ensure that others can replicate their success– all methods papers should be this detailed and helpful.<\/p>\n

The lead author, York, recently commented in the confocal listserv and shared some of his thoughts and code on the pco.edge camera (link<\/a>).<\/p>\n

\"\"<\/p>\n","protected":false},"excerpt":{"rendered":"

\n

This paper (York et al. 2012)<\/a> came out earlier this year, but I thought it’s worth highlighting here, give the subject matter.<\/p>\n

We present a hybrid technique, multifocal SIM (MSIM), that combines the resolution-doubling…<\/p>\n

Read More<\/a><\/div><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[21,36],"class_list":["post-2505","post","type-post","status-publish","format-standard","hentry","category-hardware","tag-imaging","tag-microscopy"],"_links":{"self":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/2505","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/comments?post=2505"}],"version-history":[{"count":6,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/2505\/revisions"}],"predecessor-version":[{"id":2512,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/2505\/revisions\/2512"}],"wp:attachment":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/media?parent=2505"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/categories?post=2505"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/tags?post=2505"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}