{"id":3419,"date":"2013-11-01T08:29:54","date_gmt":"2013-11-01T12:29:54","guid":{"rendered":"http:\/\/labrigger.com\/blog\/?p=3419"},"modified":"2013-11-01T08:29:54","modified_gmt":"2013-11-01T12:29:54","slug":"two-photon-technical-notes","status":"publish","type":"post","link":"http:\/\/labrigger.com\/blog\/2013\/11\/01\/two-photon-technical-notes\/","title":{"rendered":"Two photon technical notes"},"content":{"rendered":"<p align=\"center\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2012\/09\/wall.jpg\" alt=\"The figure shows the laser beam [in red, coming from the bottom right side of the panel (1)] that is first scanned by two galvanometric mirrors (2), then expanded by a telescope (3), and finally focused by the objective (4) onto the specimen (5). The emitted light (yellow) is separated from the exciting beam by a first dichroic mirror (6) and then split by a second dichroic mirror (7) in the red and green components. Two photomultipliers detect the split fluorescence emissions (8a,b).\" width=\"600\" height=\"450\" class=\"aligncenter size-full wp-image-2561\" srcset=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2012\/09\/wall.jpg 600w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2012\/09\/wall-300x225.jpg 300w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>Here&#8217;s a collection of Labrigger posts, technical notes and whatnot, on 2-photon imaging.<br \/>\n2010 &#8211; <a href=\"http:\/\/labrigger.com\/blog\/2010\/09\/23\/building-a-2-photon-microscope\/\">Building a 2-photon microscope<\/a><br \/>\n2011 &#8211; <a href=\"http:\/\/labrigger.com\/blog\/2011\/11\/11\/building-a-2p-scope-2011-edition\/\">Building a 2-photon microscope, 2011 edition<\/a><br \/>\n2010 &#8211; <a href=\"http:\/\/labrigger.com\/blog\/2010\/10\/11\/pmts-for-2-photon-imaging\/\">PMTs for 2-photon imaging<\/a><br \/>\n2011 &#8211; <a href=\"http:\/\/labrigger.com\/blog\/2011\/10\/16\/photon-counting-with-hybrid-pmts\/\">Photon counting and Hybrid PMTs<\/a><br \/>\n2011 &#8211; <a href=\"http:\/\/labrigger.com\/blog\/2011\/10\/17\/photon-counting-addendum\/\">More on photon counting<\/a><br \/>\n2010 &#8211; <a href=\"http:\/\/labrigger.com\/blog\/2010\/09\/20\/lasers-for-multiphoton-microscopy\/\">Lasers for 2-photon imaging<\/a><\/p>\n<p><a href=\"http:\/\/labrigger.com\/blog\/?s=two+photon\">More on two-photon imaging on Labrigger<\/a><\/p>\n<h3>MATLAB code<\/h3>\n<p>2011 &#8211; <a href=\"http:\/\/labrigger.com\/blog\/2011\/02\/26\/matlab-code-for-designing-collection-optics-for-2p-scopes\/\">MATLAB code for collection optics part 1<\/a><br \/>\n2011 &#8211; <a href=\"http:\/\/labrigger.com\/blog\/2011\/03\/03\/more-matlab-code-for-2p-scope-design\/\">MATLAB code for collection optics part 2<\/a><\/p>\n<h3>Calculations<\/h3>\n<p>2010 &#8211; <a href=\"http:\/\/labrigger.com\/blog\/2010\/07\/30\/measuring-the-gain-of-your-imaging-system\/\">Measuring the gain of a 2-photon imaging system<\/a><br \/>\n2010 &#8211; <a href=\"http:\/\/labrigger.com\/blog\/2010\/12\/03\/pulsed-laser-power\/\">Pulsed laser power<\/a><br \/>\n2010 &#8211; <a href=\"http:\/\/labrigger.com\/blog\/2010\/12\/15\/watts-per-photon\/\">Watts per photon<\/a><\/p>\n<h3>Some scopes available<\/h3>\n<p><a href=\"http:\/\/labrigger.com\/blog\/2013\/09\/30\/fast-resonant-scanning-two-photon-scope-from-ucla\/\">UCLA Trachtenberg Scope<\/a><br \/>\n<a href=\"http:\/\/labrigger.com\/blog\/2011\/11\/27\/thorlabs-b-scope\/\">Thorlabs B-scope<\/a><br \/>\n<a href=\"http:\/\/labrigger.com\/blog\/2012\/09\/20\/scientificas-2p-scope\/\">Scientifica<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p align=\"center\">&#8230;<\/p>\n<div class=\"read-more\"><a href=\"http:\/\/labrigger.com\/blog\/2013\/11\/01\/two-photon-technical-notes\/\">Read More<\/a><\/div><\/p>\n","protected":false},"author":1,"featured_media":2561,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[14,35,21],"class_list":["post-3419","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized","tag-construction","tag-fluorescence","tag-imaging"],"_links":{"self":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/3419","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/comments?post=3419"}],"version-history":[{"count":1,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/3419\/revisions"}],"predecessor-version":[{"id":3420,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/3419\/revisions\/3420"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/media\/2561"}],"wp:attachment":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/media?parent=3419"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/categories?post=3419"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/tags?post=3419"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}