{"id":3424,"date":"2013-11-08T21:20:44","date_gmt":"2013-11-09T02:20:44","guid":{"rendered":"http:\/\/labrigger.com\/blog\/?p=3424"},"modified":"2013-11-08T21:21:08","modified_gmt":"2013-11-09T02:21:08","slug":"sciscan-scientificas-two-photon-software","status":"publish","type":"post","link":"http:\/\/labrigger.com\/blog\/2013\/11\/08\/sciscan-scientificas-two-photon-software\/","title":{"rendered":"SciScan: Scientifica’s two photon software"},"content":{"rendered":"

\"01<\/a><\/p>\n

My friend Bruno has written some very nice software for Scientifica’s two photon microscope systems. It’s called SciScan.<\/p>\n

It’s written in LabVIEW<\/a> and runs both their conventional galvo and resonant systems. These screenshots are all from the conventional galvo version of the software, but the resonant version looks almost identical (there’s no arbitrary line scan in the resonant version, of course).<\/p>\n

\"02<\/a><\/p>\n

\"03<\/a><\/p>\n

In addition to the very basics (frame scans and z-stacks), both galvo and resonant versions can do fast volume scanning using a PIFOC piezo objective mover. Both the slow z-stacks and the fast volume scanning modes can modulate a Pockels cell with imaging depth.<\/p>\n

\"04<\/a><\/p>\n

SciScan provides Pockels cell control, PMT control and XYZ motion control and a variety of display features such as color overlay, moving averages, group averages, histograms, normalized or manual display range settings, display palettes to show saturated pixels in red, etc.<\/p>\n

\"05<\/a><\/p>\n

\"05<\/a><\/p>\n

\"06<\/a><\/p>\n

\"07<\/a><\/p>\n

For the resonance scanner, the software corrects on-the-fly for the distortion from the sinusoidal mirror velocity profile. They use a National Instrument FPGA in a PXI system for the resonant scanner data acquisition.<\/p>\n

For the conventional galvo system, the software provides arbitrary line scans, on-the-fly brightness-vs-time ROI analysis, unidirectional or bidirectional scanning, and arbitrary frames (eg. non-square pixel aspect ratio).<\/p>\n

\"08<\/a><\/p>\n

\"09<\/a><\/p>\n

\"10<\/a><\/p>\n

The data acquisition for the galvo system is based on a NI PCI 6110 card with an optional extra card to control Pockels cell and piezo.<\/p>\n

\"11<\/a><\/p>\n

For the resonance scanhead, Scientifica have completely redesigned the optical path to overfill the large back apertures of low mag, high NA objectives<\/a>. This new optical design is also available for the galvo scanhead. Alternatively, they also still have their original galvo syste<\/a>m available.<\/p>\n

Bruno will be demoing the software at the Scientifica booth at SfN in San Diego.<\/p>\n","protected":false},"excerpt":{"rendered":"

<\/a><\/p>\n

My friend Bruno has written some very nice software for Scientifica’s two photon microscope systems. It’s called SciScan.<\/p>\n

It’s written in LabVIEW<\/a> and runs both their conventional galvo and resonant…<\/p>\n

Read More<\/a><\/div><\/p>\n","protected":false},"author":1,"featured_media":3431,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6,7],"tags":[38,35,42,36,47],"class_list":["post-3424","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-hardware","category-software","tag-calcium-imaging","tag-fluorescence","tag-labview","tag-microscopy","tag-two-photon"],"_links":{"self":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/3424","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/comments?post=3424"}],"version-history":[{"count":5,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/3424\/revisions"}],"predecessor-version":[{"id":3441,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/3424\/revisions\/3441"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/media\/3431"}],"wp:attachment":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/media?parent=3424"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/categories?post=3424"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/tags?post=3424"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}