{"id":4118,"date":"2014-12-30T18:23:27","date_gmt":"2014-12-30T23:23:27","guid":{"rendered":"http:\/\/labrigger.com\/blog\/?p=4118"},"modified":"2014-12-30T18:23:27","modified_gmt":"2014-12-30T23:23:27","slug":"olympus-10x-for-two-photon-microscopy","status":"publish","type":"post","link":"http:\/\/labrigger.com\/blog\/2014\/12\/30\/olympus-10x-for-two-photon-microscopy\/","title":{"rendered":"Olympus 10x for two-photon microscopy"},"content":{"rendered":"<p align=\"center\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2014\/12\/obj.png\" alt=\"obj\" width=\"407\" height=\"560\" class=\"alignnone size-full wp-image-4119\" srcset=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2014\/12\/obj.png 407w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2014\/12\/obj-218x300.png 218w\" sizes=\"auto, (max-width: 407px) 100vw, 407px\" \/><\/p>\n<p>Olympus released a suite of new objectives this year, and one of them is <a href=\"http:\/\/microscope.olympus-global.com\/uis2\/en\/xlpln10xsvmp\/\">this 10x\/0.6 NA<\/a>. This is probably the longest focal length (180\/10 = 18 mm) objective you can get and use with a standard two-photon system and see activity in single neurons. <\/p>\n<p>0.6 NA might not be enough for tissue bulk labeled with OGB-1, but for GECIs like GCaMP6, signals from single cells are easily resolved. The supplement to the recent <a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25402858\">Lecoq et al. paper<\/a> has some analysis of how relatively moderate NAs can yield single cell resolution. Some commercial rigs underfill the 16x\/0.8 NAs already, and are probably using something more like 0.6-0.7 NA already.<\/p>\n<p>The field of view isn&#8217;t going to be <a href=\"http:\/\/labrigger.com\/blog\/2014\/11\/23\/field-of-view-3-5-mm-with-cellular-resolution\/\">3.5 mm wide<\/a>, of course. That takes more extensive customization. But for conventional systems, this objective is probably the best bet. Albeit a 5-figure bet.<\/p>\n<p align=\"center\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2014\/12\/msmt.png\" alt=\"msmt\" width=\"800\" height=\"563\" class=\"alignnone size-full wp-image-4120\" srcset=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2014\/12\/msmt.png 800w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2014\/12\/msmt-300x211.png 300w\" sizes=\"auto, (max-width: 800px) 100vw, 800px\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p align=\"center\">\n<p>Olympus released a suite of new objectives this year, and one of them is <a href=\"http:\/\/microscope.olympus-global.com\/uis2\/en\/xlpln10xsvmp\/\">this 10x\/0.6 NA<\/a>. This is probably the longest focal length (180\/10 = 18 mm) objective you can get and use&#8230;<\/p>\n<div class=\"read-more\"><a href=\"http:\/\/labrigger.com\/blog\/2014\/12\/30\/olympus-10x-for-two-photon-microscopy\/\">Read More<\/a><\/div><\/p>\n","protected":false},"author":1,"featured_media":4119,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[38,21,36,15],"class_list":["post-4118","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-hardware","tag-calcium-imaging","tag-imaging","tag-microscopy","tag-optics"],"_links":{"self":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/4118","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/comments?post=4118"}],"version-history":[{"count":4,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/4118\/revisions"}],"predecessor-version":[{"id":4125,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/4118\/revisions\/4125"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/media\/4119"}],"wp:attachment":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/media?parent=4118"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/categories?post=4118"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/tags?post=4118"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}