{"id":4344,"date":"2015-11-26T13:22:38","date_gmt":"2015-11-26T18:22:38","guid":{"rendered":"http:\/\/labrigger.com\/blog\/?p=4344"},"modified":"2015-11-26T13:23:25","modified_gmt":"2015-11-26T18:23:25","slug":"intersectional-strategies-cre-dog-and-so-forth","status":"publish","type":"post","link":"http:\/\/labrigger.com\/blog\/2015\/11\/26\/intersectional-strategies-cre-dog-and-so-forth\/","title":{"rendered":"Intersectional strategies: CRE-DOG"},"content":{"rendered":"<p align=\"center\">\n<a href=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2015\/11\/cre-dog.png\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2015\/11\/cre-dog.png\" alt=\"cre-dog\" width=\"444\" height=\"181\" class=\"alignnone size-full wp-image-4345\" srcset=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2015\/11\/cre-dog.png 444w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2015\/11\/cre-dog-300x122.png 300w\" sizes=\"auto, (max-width: 444px) 100vw, 444px\" \/><\/a><\/p>\n<p>Intersectional strategies are rapidly growing in sophistication. Unfortunately, it still takes time to generate mice and make crosses as needed, so short cuts are handy.<\/p>\n<p>The CRE-DOG technique, from Connie Cepko&#8217;s lab, induces expression in cells that are already expressing GFP. No Cre needed. If you have a fusion protein line or a BAC transgenic or something similar, you can combine it with this approach to introduce a transgene to GFP-expressing cells.<br \/>\nI asked Connie about potential crosstalk&#8230;<\/p>\n<blockquote><p>we looked into reactivity with gfp derivatives, yfp and cfp. it does recognize those, as expected. for tdtomato and other coral red derivatives, it does not. not sure about gcamp.<\/p><\/blockquote>\n<p><a href=\"https:\/\/www.addgene.org\/browse\/article\/15732\/\">Parts on Addgene<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p align=\"center\">\n<a href=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2015\/11\/cre-dog.png\"><\/a><\/p>\n<p>Intersectional strategies are rapidly growing in sophistication. Unfortunately, it still takes time to generate mice and make crosses as needed, so short cuts are handy.<\/p>\n<p>The CRE-DOG technique, from Connie Cepko&#8217;s lab, induces expression in&#8230;<\/p>\n<div class=\"read-more\"><a href=\"http:\/\/labrigger.com\/blog\/2015\/11\/26\/intersectional-strategies-cre-dog-and-so-forth\/\">Read More<\/a><\/div><\/p>\n","protected":false},"author":1,"featured_media":4345,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-4344","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"_links":{"self":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/4344","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/comments?post=4344"}],"version-history":[{"count":6,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/4344\/revisions"}],"predecessor-version":[{"id":4352,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/4344\/revisions\/4352"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/media\/4345"}],"wp:attachment":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/media?parent=4344"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/categories?post=4344"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/tags?post=4344"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}