{"id":451,"date":"2010-08-17T04:17:16","date_gmt":"2010-08-17T04:17:16","guid":{"rendered":"http:\/\/labrigger.com\/blog\/?p=451"},"modified":"2010-08-16T11:31:06","modified_gmt":"2010-08-16T11:31:06","slug":"troubleshooting-fluorescence-microscopy","status":"publish","type":"post","link":"http:\/\/labrigger.com\/blog\/2010\/08\/17\/troubleshooting-fluorescence-microscopy\/","title":{"rendered":"Troubleshooting Fluorescence Microscopy"},"content":{"rendered":"<p align=\"center\"><a href=\"http:\/\/www.love39steps.com\/\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2010\/08\/39steps.png\" alt=\"\" title=\"39steps\" width=\"430\" height=\"392\" class=\"aligncenter size-full wp-image-458\" srcset=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2010\/08\/39steps.png 430w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2010\/08\/39steps-300x273.png 300w\" sizes=\"auto, (max-width: 430px) 100vw, 430px\" \/><\/a><\/p>\n<p>Jim Pawley upgraded the 39 Steps from a simple <a href=\"http:\/\/en.wikipedia.org\/wiki\/MacGuffin\">MacGuffin<\/a> to a classic checklist for troubleshooting fluorescent microscopy images. Now 10 years old, it remains very relevant. It covers thirty-nine different parameters to check: from singlet state saturation to embedding medium. It&#8217;s a good place to start if you&#8217;re puzzled as to why your images don&#8217;t look as good as you expected.<br \/>\n<a href=\"http:\/\/labs.pbrc.edu\/cellbiology\/documents\/39steps.pdf\">Jim Pawley&#8217;s <em>The 39 Steps<\/em><\/a> (thanks to CW for the tip)<\/p>\n<p>On a related note, if you&#8217;re in London, the stage production of <em>The 39 Steps<\/em> at the Criterion theater is a great comedy to check out.<\/p>\n<p>BTW, here are the online microscopy and imaging references from the major microscope manufacturers. These can also help in troubleshooting.<br \/>\n<a href=\"http:\/\/www.olympusmicro.com\/\">Olympus Microscopy Resource Center<br \/>\n<\/a><a href=\"http:\/\/www.microscopyu.com\/\">Nikon&#8217;s Microscopy U<\/a><br \/>\n<a href=\"http:\/\/zeiss-campus.magnet.fsu.edu\/index.html\">Carl Zeiss MicroImaging Online Campus<\/a><\/p>\n<p>Don&#8217;t forget <a href=\"http:\/\/labrigger.com\/blog\/2010\/07\/01\/catalogs-as-textbooks\/\">catalogs as textbooks<\/a>!<\/p>\n","protected":false},"excerpt":{"rendered":"<p align=\"center\"><a href=\"http:\/\/www.love39steps.com\/\"><\/a><\/p>\n<p>Jim Pawley upgraded the 39 Steps from a simple <a href=\"http:\/\/en.wikipedia.org\/wiki\/MacGuffin\">MacGuffin<\/a> to a classic checklist for troubleshooting fluorescent microscopy images. Now 10 years old, it remains very relevant. It covers thirty-nine different parameters&#8230;<\/p>\n<div class=\"read-more\"><a href=\"http:\/\/labrigger.com\/blog\/2010\/08\/17\/troubleshooting-fluorescence-microscopy\/\">Read More<\/a><\/div><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[21,20],"class_list":["post-451","post","type-post","status-publish","format-standard","hentry","category-tips","tag-imaging","tag-references"],"_links":{"self":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/451","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/comments?post=451"}],"version-history":[{"count":13,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/451\/revisions"}],"predecessor-version":[{"id":465,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/451\/revisions\/465"}],"wp:attachment":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/media?parent=451"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/categories?post=451"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/tags?post=451"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}