{"id":4921,"date":"2018-12-23T10:27:08","date_gmt":"2018-12-23T15:27:08","guid":{"rendered":"http:\/\/labrigger.com\/blog\/?p=4921"},"modified":"2018-12-23T10:27:08","modified_gmt":"2018-12-23T15:27:08","slug":"open-easy-laser-scanning-microscope-software-lsmaq","status":"publish","type":"post","link":"http:\/\/labrigger.com\/blog\/2018\/12\/23\/open-easy-laser-scanning-microscope-software-lsmaq\/","title":{"rendered":"Open, easy laser-scanning microscope software: LSMAQ"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2018\/12\/gui-1024x447.png\" alt=\"\" width=\"757\" height=\"330\" class=\"aligncenter size-large wp-image-4922\" srcset=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2018\/12\/gui-1024x447.png 1024w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2018\/12\/gui-300x131.png 300w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2018\/12\/gui-768x336.png 768w\" sizes=\"auto, (max-width: 757px) 100vw, 757px\" \/><\/p>\n<p>Years ago, <a href=\"https:\/\/www.jlab.berlin\/\">Benjamin Judkewitz<\/a> wanted to try some new techniques out with laser scanning two-photon microscopy. However, the software we were using was rather cumbersome to modify. So he wrote his own software from scratch (which took him roughly one day). The result was <a href=\"https:\/\/github.com\/danionella\/lsmaq\">lsmaq<\/a>.<\/p>\n<blockquote><p>LSMAQ is a lightweight and flexible laser scanning microscope acquisition software written in MATLAB. It supports National Instruments hardware for galvo-based scanning.<\/p><\/blockquote>\n<p>It does the essentials: it sends scan mirror commands out, reads image data in, and makes images. It has a live view and can stream to disk. He worked from that baseline to create the modified versions he wanted. It was a useful starting point for other people in the lab as well. <a href=\"http:\/\/www.ucl.ac.uk\/swc\/research\/Branco\">Tiago Branco<\/a> used it as a basis for two-photon uncaging software. <a href=\"http:\/\/slslab.org\/\">I<\/a> used it for arbitrary line scanning software in dendritic patch and imaging experiments. (Tiago, Ben, and I all overlapped in the Hausser Lab.)<\/p>\n<p>It&#8217;s also useful for learning. There are relatively few lines of code, and the layout is straightforward.<\/p>\n<p>Now, it is publicly available on GitHub, thanks to Ben.<br \/>\n<a href=\"https:\/\/github.com\/danionella\/lsmaq\">https:\/\/github.com\/danionella\/lsmaq<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<\/p>\n<p>Years ago, <a href=\"https:\/\/www.jlab.berlin\/\">Benjamin Judkewitz<\/a> wanted to try some new techniques out with laser scanning two-photon microscopy. However, the software we were using was rather cumbersome to modify. So he wrote his own software&#8230;<\/p>\n<div class=\"read-more\"><a href=\"http:\/\/labrigger.com\/blog\/2018\/12\/23\/open-easy-laser-scanning-microscope-software-lsmaq\/\">Read More<\/a><\/div><\/p>\n","protected":false},"author":1,"featured_media":4922,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[38,21,26,36,47],"class_list":["post-4921","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-software","tag-calcium-imaging","tag-imaging","tag-matlab","tag-microscopy","tag-two-photon"],"_links":{"self":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/4921","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/comments?post=4921"}],"version-history":[{"count":1,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/4921\/revisions"}],"predecessor-version":[{"id":4923,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/4921\/revisions\/4923"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/media\/4922"}],"wp:attachment":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/media?parent=4921"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/categories?post=4921"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/tags?post=4921"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}