{"id":5019,"date":"2020-11-10T10:46:23","date_gmt":"2020-11-10T15:46:23","guid":{"rendered":"http:\/\/labrigger.com\/blog\/?p=5019"},"modified":"2020-11-10T10:46:25","modified_gmt":"2020-11-10T15:46:25","slug":"a-20-mm-working-distance-objective-for-two-photon-imaging","status":"publish","type":"post","link":"http:\/\/labrigger.com\/blog\/2020\/11\/10\/a-20-mm-working-distance-objective-for-two-photon-imaging\/","title":{"rendered":"A 20 mm working distance objective for two-photon imaging"},"content":{"rendered":"\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"623\" height=\"303\" src=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2020\/11\/bb-diag.png\" alt=\"\" class=\"wp-image-5020\" srcset=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2020\/11\/bb-diag.png 623w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2020\/11\/bb-diag-300x146.png 300w\" sizes=\"auto, (max-width: 623px) 100vw, 623px\" \/><figcaption>The working distance is marked as 22 mm in this image, which is measured on-axis, from the center of the last lens. Practically, the mechanical working distance will be 20 mm.<\/figcaption><\/figure>\n\n\n\n<p>We have designed a 20 mm working distance air immersion objective for multiphoton imaging. The target application is for surgical preparations that make it difficult to use conventional objectives with shorter working distances and\/or a requirement for a water interface. This objective is all about getting the geometry to work out. If you&#8217;re frustrated with the working distance limitations of objectives on the market for your particular surgical prep, we hope this can be an interesting solution for your work.<\/p>\n\n\n\n<p>We already have some early adopters and we&#8217;re going to have a first run made soon. <strong>If you want in, contact us by the end of the day this Friday (Nov 13, 2020).<\/strong> We can accommodate some more people. $15k is the price.<\/p>\n\n\n\n<p><strong>Specifications<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td>Design wavelength range<\/td><td>920 nm \u00b1 10 nm<\/td><\/tr><tr><td>Working distance<\/td><td>20 mm<\/td><\/tr><tr><td>Numerical aperture<\/td><td>0.5<\/td><\/tr><tr><td>Magnification<\/td><td>10 X (with 200-mm focal length tube lens)<\/td><\/tr><tr><td>Focal length<\/td><td>20 mm<\/td><\/tr><tr><td>Field of view<\/td><td>2.08 mm (diameter)<\/td><\/tr><tr><td>Scanning angles<\/td><td>\u00b1 3 degrees<\/td><\/tr><tr><td>Entrance pupil<\/td><td>20 mm (diameter)<\/td><\/tr><tr><td>Parfocal distance<\/td><td>~90 mm<\/td><\/tr><tr><td>Mounting thread size<\/td><td>M32 x 0.75<\/td><\/tr><tr><td>Immersion media<\/td><td>Air<\/td><\/tr><tr><td>Coverslip thickness<\/td><td>Correction collar (0 &#8211; 1 mm glass thickness)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"480\" height=\"271\" src=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2020\/11\/sr-1.png\" alt=\"\" class=\"wp-image-5022\" srcset=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2020\/11\/sr-1.png 480w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2020\/11\/sr-1-300x169.png 300w\" sizes=\"auto, (max-width: 480px) 100vw, 480px\" \/><figcaption>Strehl ratio (anything over 0.8 is good) across the field of view as measured in scan angle (degrees) for three different wavelengths (910 nm, 920 nm, 930 nm).<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"480\" height=\"272\" src=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2020\/11\/rms-we.png\" alt=\"\" class=\"wp-image-5023\" srcset=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2020\/11\/rms-we.png 480w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2020\/11\/rms-we-300x170.png 300w\" sizes=\"auto, (max-width: 480px) 100vw, 480px\" \/><figcaption>RMS wavefront error (anything under the black line is good) across the field of view as a function of scan angle (degrees) for three different wavelengths (910 nm, 920 nm, 930 nm).<\/figcaption><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>The working distance is marked as 22 mm in this image, which is measured on-axis, from the center of the last lens. Practically, the mechanical working distance will be 20 mm.<\/p>\n<p>We have designed a 20 mm&#8230;<\/p>\n<div class=\"read-more\"><a href=\"http:\/\/labrigger.com\/blog\/2020\/11\/10\/a-20-mm-working-distance-objective-for-two-photon-imaging\/\">Read More<\/a><\/div><\/p>\n","protected":false},"author":1,"featured_media":5020,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[36,15,47],"class_list":["post-5019","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-hardware","tag-microscopy","tag-optics","tag-two-photon"],"_links":{"self":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/5019","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/comments?post=5019"}],"version-history":[{"count":1,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/5019\/revisions"}],"predecessor-version":[{"id":5024,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/5019\/revisions\/5024"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/media\/5020"}],"wp:attachment":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/media?parent=5019"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/categories?post=5019"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/tags?post=5019"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}