{"id":5171,"date":"2023-05-02T15:14:03","date_gmt":"2023-05-02T19:14:03","guid":{"rendered":"http:\/\/labrigger.com\/blog\/?p=5171"},"modified":"2023-05-02T16:02:36","modified_gmt":"2023-05-02T20:02:36","slug":"when-do-you-need-a-pulse-compressor-for-multiphoton-imaging","status":"publish","type":"post","link":"http:\/\/labrigger.com\/blog\/2023\/05\/02\/when-do-you-need-a-pulse-compressor-for-multiphoton-imaging\/","title":{"rendered":"When do you need a pulse compressor for multiphoton imaging?"},"content":{"rendered":"\n<figure class=\"wp-block-image size-large\"><a href=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2023\/05\/image-1.png\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"759\" src=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2023\/05\/image-1-1024x759.png\" alt=\"\" class=\"wp-image-5173\" srcset=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2023\/05\/image-1-1024x759.png 1024w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2023\/05\/image-1-300x222.png 300w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2023\/05\/image-1-768x569.png 768w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2023\/05\/image-1.png 1430w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><figcaption>There are different types of pulse compressors, this is clever single prism design.<\/figcaption><\/figure>\n\n\n\n<p>Here&#8217;s a quick question I received via email that might be worth sharing: <em>Does using a pulse compressor make a noticeable difference?<\/em> <\/p>\n\n\n\n<p>It can, specifically when:<\/p>\n\n\n\n<p>(1) There is a lot of glass in the system (more than a typical system&#8211; our Diesel2p system needs it),<br>(2) You&#8217;re starting with very short pulses (e.g., &lt;100 fs),<br>and\/or<br>(3) You&#8217;re working right at the limit of average power (e.g., 100 mW is too dim, but 120 mW damages tissue)<\/p>\n\n\n\n<p>In general, <strong>shorter<\/strong> <strong>pulses<\/strong> suffer more dispersion (because they typically have larger spectral bandwidth), and <strong>shorter<\/strong> <strong>wavelengths<\/strong> suffer more dispersion (because the index of refraction typically changes faster, per nm of wavelength, towards the blue\/uv end of the spectrum). <\/p>\n\n\n\n<p>So if you&#8217;re imaging with 1050 nm, starting with ~150 fs pulses, and you only need 20 mW to image and your damage threshold is closer to 200 mW, and you don&#8217;t have much glass in your system (i.e., a conventional system), then a pulse compressor might not buy you much.<\/p>\n\n\n\n<p>Many laser systems, including Ti:Sapphs and fiber lasers, can be purchased with built-in compressors, and these have generally worked quite well. There are standalone pulse-compressors too. If you want to learn more about them, <a href=\"https:\/\/www.swampoptics.com\/pulse-compressors.html\">Swamp Optics<\/a> has excellent info on their web site.<\/p>\n\n\n\n<p>Thanks, Rick Trebino:<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2023\/05\/image.png\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"570\" src=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2023\/05\/image-1024x570.png\" alt=\"\" class=\"wp-image-5172\" srcset=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2023\/05\/image-1024x570.png 1024w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2023\/05\/image-300x167.png 300w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2023\/05\/image-768x428.png 768w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2023\/05\/image.png 1092w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><\/figure>\n","protected":false},"excerpt":{"rendered":"<p><a href=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2023\/05\/image-1.png\"><\/a>There are different types of pulse compressors, this is clever single prism design.<\/p>\n<p>Here&#8217;s a quick question I received via email that might be worth sharing: <em>Does using a pulse compressor make&#8230;<\/p>\n<div class=\"read-more\"><a href=\"http:\/\/labrigger.com\/blog\/2023\/05\/02\/when-do-you-need-a-pulse-compressor-for-multiphoton-imaging\/\">Read More<\/a><\/div>\n<p><\/em><\/p>\n","protected":false},"author":1,"featured_media":5173,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-5171","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"_links":{"self":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/5171","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/comments?post=5171"}],"version-history":[{"count":2,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/5171\/revisions"}],"predecessor-version":[{"id":5175,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/5171\/revisions\/5175"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/media\/5173"}],"wp:attachment":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/media?parent=5171"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/categories?post=5171"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/tags?post=5171"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}