{"id":834,"date":"2010-11-06T00:53:12","date_gmt":"2010-11-06T00:53:12","guid":{"rendered":"http:\/\/labrigger.com\/blog\/?p=834"},"modified":"2010-11-06T00:53:12","modified_gmt":"2010-11-06T00:53:12","slug":"cameras-for-ultra-low-light-levels","status":"publish","type":"post","link":"http:\/\/labrigger.com\/blog\/2010\/11\/06\/cameras-for-ultra-low-light-levels\/","title":{"rendered":"Cameras for ultra low light levels"},"content":{"rendered":"<p align=\"center\">\n<a href=\"http:\/\/www.i-micronews.com\/news\/Sony-Backside-Illuminated-BSI-CMOS-Image-Sensor,3161.html\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2010\/11\/Backside.jpg\" alt=\"\" title=\"Back illuminated sensor cross-section from Sony\" width=\"429\" height=\"301\" class=\"aligncenter size-full wp-image-854\" srcset=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2010\/11\/Backside.jpg 429w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2010\/11\/Backside-300x210.jpg 300w\" sizes=\"auto, (max-width: 429px) 100vw, 429px\" \/><\/a>\n<\/p>\n<p>Many image sensors are built backwards, just like the retina. The actual photon detectors are buried behind a mesh of circuitry. Back-illuminated sensors, where the detectors are put in front, were a big step forward for light-limited applications. Now they&#8217;re so common, even the iPhone 4 has a back-illuminated CMOS camera. A couple of other big steps forward were intensified CCDs and electron multiplying CCDs.<\/p>\n<p>In electron multiplying CCDs (<a href=\"http:\/\/en.wikipedia.org\/wiki\/Charge-coupled_device#Electron-multiplying_CCD\">EMCCDs<\/a>) (<a href=\"http:\/\/www.emccd.com\/what_is_emccd\/emccd_tutorial\/\">some nice tutorials<\/a>) the signal is amplified on chip, just prior to reading. So read noise is vastly improved. Of course, the dark noise is also amplified, but in practice this hasn&#8217;t been a large limitation. At low light levels, their main competitor is intensified CCDs (ICCDs). When imaging fast, and with around 10 photons\/pixel, it&#8217;s a hard call whether to go with an ICCD or an EMCCD. The EMCCD needs aggressive cooling and does better with slower frame rates, but the ICCD has only a 50% QE compared to the >90% QE of EMCCDs. There are some analyses of the signal-to-noise ratio under different circumstances in ICCDs and EMCCDs (e.g., <a href=\"http:\/\/www.emccd.com\/what_is_emccd\/emccd_vs_other_detector_types\/EMCCD_vs_ICCD\/\">this<\/a>, <a href=\"http:\/\/www.andor.com\/learning\/digital_cameras\/?docid=327\">this<\/a>, and <a href=\"http:\/\/www.stanfordcomputeroptics.com\/PDF\/noise-performance-comparison-iccd-ccd-emccd.pdf\">this pdf<\/a>), but it&#8217;s a thorny issue. I recommend you demo both. Also keep in mind that EMCCDs age in response to usage of the high gain modes (<a href=\"http:\/\/www.e2v.com\/assets\/media\/files\/documents\/imaging-space-and-scientific-sensors\/Papers\/low-light_TN5.pdf\">pdf<\/a>).<\/p>\n<p align=\"center\"><a href=\"http:\/\/www.emccd.com\/what_is_emccd\/emccd_vs_other_detector_types\/EMCCD_vs_ICCD\/\"><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2010\/11\/EMCCDvsICCD.gif\" alt=\"\" title=\"(A) Comparative raw and smoothed low-light fluorescent images recorded of a live smooth muscle cell using the BV-EMCCD and the Gen III+ ICCD (B) Further comparative cell images of weakly loaded cell, representative of an image close to the \u2018photon shot noise\u2019 detection limit; 33 ms exposure time per frame; high EMCCD or ICCD gain setting throughout to eliminate read noise.\" width=\"428\" height=\"722\" class=\"aligncenter size-full wp-image-843\" srcset=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2010\/11\/EMCCDvsICCD.gif 428w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2010\/11\/EMCCDvsICCD-177x300.gif 177w\" sizes=\"auto, (max-width: 428px) 100vw, 428px\" \/><\/a><\/p>\n<p>These products aren&#8217;t to market yet, but they&#8217;re something to keep an eye on: micro single photon detectors. Philips has an <a href=\"http:\/\/www.research.philips.com\/initiatives\/digitalphotoncounting\/\">8&#215;8 array<\/a> of avalanche photodiodes, which has on-chip amplifiers and photon counting. Hamamatsu has <a href=\"http:\/\/www.hamamatsu.com\/news\/2010\/2010_09_28.html\">a MEMS PMT<\/a>. Among other things, this technology raises the possibility of an image sensor with 120 dB of dynamic range.<\/p>\n<p align=\"center\">\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2010\/11\/H_MEMSPMT3.png\" alt=\"\" title=\"Hamamatsu microPMT\" width=\"400\" height=\"293\" class=\"aligncenter size-full wp-image-849\" srcset=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2010\/11\/H_MEMSPMT3.png 400w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2010\/11\/H_MEMSPMT3-300x219.png 300w\" sizes=\"auto, (max-width: 400px) 100vw, 400px\" \/><br \/>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2010\/11\/P_PMTARRAY1.jpg\" alt=\"\" title=\"Philips PMT array\" width=\"560\" height=\"250\" class=\"aligncenter size-full wp-image-846\" srcset=\"http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2010\/11\/P_PMTARRAY1.jpg 560w, http:\/\/labrigger.com\/blog\/wp-content\/uploads\/2010\/11\/P_PMTARRAY1-300x133.jpg 300w\" sizes=\"auto, (max-width: 560px) 100vw, 560px\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p align=\"center\">\n<a href=\"http:\/\/www.i-micronews.com\/news\/Sony-Backside-Illuminated-BSI-CMOS-Image-Sensor,3161.html\"><\/a>\n<\/p>\n<p>Many image sensors are built backwards, just like the retina. The actual photon detectors are buried behind a mesh of circuitry. Back-illuminated sensors, where the detectors are put&#8230;<\/p>\n<div class=\"read-more\"><a href=\"http:\/\/labrigger.com\/blog\/2010\/11\/06\/cameras-for-ultra-low-light-levels\/\">Read More<\/a><\/div><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[31,21],"class_list":["post-834","post","type-post","status-publish","format-standard","hentry","category-hardware","tag-equipment","tag-imaging"],"_links":{"self":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/834","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/comments?post=834"}],"version-history":[{"count":20,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/834\/revisions"}],"predecessor-version":[{"id":859,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/posts\/834\/revisions\/859"}],"wp:attachment":[{"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/media?parent=834"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/categories?post=834"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/labrigger.com\/blog\/wp-json\/wp\/v2\/tags?post=834"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}