Enrico Stefani’s lab at UCLA has a couple of crack engineers on board, Yong Wu and Pedro Felipe Gardeazábal Rodríguez. These two fellows built a custom STED system and have documented the setup on …
Drosophila embryos are a fraction of a square mm, and go from fertilization to hatching (as a larva) in about 22 hrs. So it’s possible to image individual embryos in their entirety with…
To recap the previous post on axial resolution and numerical aperture in two-photon microscopy:
For excitation deep in scattering tissue, higher NA can actually be detrimental because the light cone…
Recently, microscope manufacturers have been releasing ever higher NA objectives for multiphoton imaging. Although higher NA objectives should give better axial resolution, they might not be ideal for imaging deep into…
Roger Tsien’s lab recently published the new generation voltage sensitive dye they were presenting at SfN: VoltageFluors. As often when then Tsien Lab takes on a new field, they start by taking a…
Zeiss released an iOS app for viewing spectra.
No Android version yet, but Johannes Amon said:
of course I can’t tell you any specifics but at the moment we are evaluating a…
Let me add some clarification to the hybrid PMT post.
The actual signal that comes off of a PMT in response to a photon is a pulse of…
George McNamara is planning on updating the massive PubSpectra (previously mentioned here).
He’s currently soliciting submissions. Do you have any to contribute?
(link)
…
Hybrid PMTs have made their way into fluorescence microscopy and are competing with GaAsP PMTs. (Well, actually, Hamamatsu makes both types, so it’s not really competition…
Post by Christian Wilms
Call me old-fashioned, but I’m a big fan of small organic calcium indicators (e.g., Oregon Green BAPTA 1). Yes, genetically encoded calcium indicators (GECIs) have many…
While on the Gnotero page (a Python app for accessing refs from Zotero, a Firefox plugin-based citation manager, in case you’re wondering), I…
On the off chance that you missed it, Drobizhev et al. published a bunch of 2p cross sections for fluorescent proteins.
Earlier:
2p cross sections
More…
This is a cute little product that lets you retrofit a standard microscope for fluorescence. It contains an LED light source, dichroic, and filters. It may be clumsy to have this big blue cannon…
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