There are some companies that have no real competition. Maybe a product here-and-there in their catalog is subject to market forces, but most of their items have no competing options from other companies….
The working distance is marked as 22 mm in this image, which is measured on-axis, from the center of the last lens. Practically, the mechanical working distance will be 20 mm.
We have designed a 20 mm…
Years ago, Benjamin Judkewitz wanted to try some new techniques out with laser scanning two-photon microscopy. However, the software we were using was rather cumbersome to modify. So he wrote his own software…
This could be handy for some of you. Resonant scanners are fast, but inflexible. You can’t tell them where to go. They just oscillate at a fixed frequency. All you can do is control…
Frontiers in BioImaging 2018 will take place on Wednesday 27 and Thursday 28 June at the Technology and Innovation Centre, Glasgow. It will focus on the latest developments in applications of…
Andre and colleagues have a preprint up on their project generating inexpensive instrumentation for imaging and optogenetics experiments. The 100 Euro Lab: A 3-D Printable Open Source Platform For Fluorescence…
Reminder: green laser pointers have a ton of IR in them. It needs to be filtered out if you don’t want it. See this old (2011!) Labrigger post.
PMT performance can degrade over time. A friend asked mine recently ask for suggestions on how to check PMT performance. In a prior post, I mentioned that the Hamamatsu PMT Handbook talks…
Peng Xi (Peking University) shared this resource his lab has developed: software for processing images for structured illumination (a superresolution technique).
Here’s the Github repository.
And here’s the paper…
Bar-Noam et al. 2016 (Shy Shoham’s lab) figured out how to image the mouse retina with 2-photon microscopy in vivo.
…
The UCLA Miniscope project is an NIH BRAIN Initiative-funded project to open source head-mounted calcium imaging devices. Their web site is online now. They’ll be releasing all of the information needed for making these devices yourself, including data analysis. They’ll also…
Trying to decide which optical design software to use? Just try them out and decide for yourself. I like how Steve Eckhardt (great guy, btw, in case you’re looking for someone to consult…
Austin Blanco has designed and built an open-source system for controlling complex imaging systems called TriggerScope. It’s highly customizable out of the box, and both the firmware and software are open.
Olympus released a suite of new objectives this year, and one of them is this 10x/0.6 NA. This is probably the longest focal length (180/10 = 18 mm) objective you can get and use…
In this preprint, Stirman et al. report achieving a 3.5 mm field of view with 2-photon excitation with cellular resolution. The design involves custom scan optics and a custom objective built in the…
