Tag: imaging

100 billion frames per s

Yes, that’s what you think it is. Courtesy of a compressed sensing + streak camera rig made by Liang Gao, Jinyang Liang, Chiye Li & Lihong V. Wang.

Link to video.



Field of view = 3.5 mm with cellular resolution

In this preprint, Stirman et al. report achieving a 3.5 mm field of view with 2-photon excitation with cellular resolution. The design involves custom scan optics and a custom objective built in the SLAB….



Lightfield imaging – development kit from Lytro

Lytro is releasing a development kit for their light field camera. That’s nice, but it pretty expensive. And we’ve been able to buy light field cameras for technical uses for years.

What’s special…



Rolling shutter vs. global shutter: why it matters

Digital cameras can have global shutters or rolling shutters (some cameras can operate in both modes). Global shutters are preferable for periodic applications, including intrinsic imaging, because rolling shutters can cause artifacts. This article does a nice job of explaining the artifacts…



Resonant scanning with ScanImage 5

ScanImage 5 supports resonant scanning with a wide range of hardware. So custom rigs can add resonant scanning pretty easily, while sticking with ScanImage for acquisition.

It takes about $10,000 worth of electronics from…



Notes from Austin Blanco

Our friend Christian Wilms tipped us to Austin Blanco’s blog, which has some posts you all might be interested in:

Characterizing unknown optical components
A few notes on Arduinos, their timers, and using them…



ImageSurfer 64-bit

UNC-Chapel Hill’s Computer Integrated Systems for Microscopy and Manipulation team released a new version of their popular ImageSurfer software. All 64-bit, with versions for Linux, OSX, and Windows.



What’s your favorite LUT?

Parula is the new default colormap for MATLAB (namesake above, actual map below). It probably collapses to grey better than jet (which is good for colorblind readers).

You aficionados care dearly about …



The commoditization of image sensors

About 25 years ago, pro-level audio recording technology was prohibitively expensive for home studios. Then, digital audio technology like the Alesis ADAT enabled working class musicians to produce high fidelity recordings. The fast…



Custom scan optics for two-photon imaging

Adrian Negrean, in Huibert Mansvelder’s lab, has been designing and building custom scan optics for two-photon imaging and earlier this year published his work (paper). Labrigger wants to emphasize how nice this paper is. It’s…



SfN Short Courses – Advanced techniques

Sign up now for SfN Short Courses. In addition to the excellent material that is covered, these workshops can be a great opporunity to make new connections with like-minded people– scientists with the similar…



Optomechanics and microscopy technical notes – many items

Vojnovic’s group at Oxford has dozens of technical notes. Most are concise, and include software code, if applicable. SolidWorks files and PCB files are available on request.

Here are a few examples:

A motorized 4-way…



Meyer Navigator for ScanImage

Software from Stephan Meyer Zum Alten (Gilbert lab, Rockefeller):

I put together a sort of Navigator for Janelia Farms ScanImage Two Photon control software, which we use a lot in our lab. There was no way…



Over 100 DIY pieces of lab equipment

Labrigger previously mentioned this contest. All of the 173 projects are online for your perusal. See if any of them inspire you.



ScanBox – free, open, MATLAB-based software for two-photon microscopy

Dario Ringach has written some nice software for the Trachtenberg scope mentioned before on Labrigger. They also have put together their own Cypress PSoC-based hardware box to control several parts of the system.

He set…