Tag: imaging

ACQ4: A Python-based open source system for neurophysiology

Luke Campagnola, Megan Kratz, and Paul Manis recently published their in-house software for neurophysiology experiments. It’s an extensive set of tools, including multiphoton imaging, photostimulation mapping, image mosaic construction, electrophysiology, and more.

Website: acq4.org



Two photon technical notes



Visual stimulation and intrinsic imaging code

Ian Nauhaus, whom UT Austin recently nabbed, is sharing his code for intrinsic imaging and visual stimulation.

The visual stimulation code is based off of Psychophysics Toolbox, which is already in use…



OpenSPIM and OpenSpinMicroscopy for light sheet microscopy

This month, Nature Methods highlights a couple of open light sheet microscopy…



MATLAB 2013a natively supports Kinect

There have been wrappers for interfacing with the Kinect device before, but the latest update to MATLAB makes it native to the Image Processing Toolbox. source

Drop a Kinect…



Scattering in tissue

Post by Jeffrey Stirman

The opacity of the brain is one barrier to optically imaging individual neurons and their connections. Scattering in tissue is the main reason tissue is not transparent; absorption also plays a…



Commoditizing population calcium imaging analysis

More open source software to check out.

Two-Photon Processor and SeNeCA – A freely available…



EMCCD Specs

Editorial preface: Labrigger takes submissions from anyone. We post them if they’re potentially useful. In this case, we received some material from a commercial source. Labrigger is not endorsing their products. But we recognize…



Interview with the developers of HelioScan

Last Monday, Labrigger covered HelioScan, a LabVIEW-based, two-photon laser scanning microscopy software suite.

Marcel van ‘t…



Scientifica’s 2p scope

With its newly introduced scan head, Scientifica’s 2p scope is finally a complete package. They’re happy to sell their modular design in pieces, so this fills the void between fully custom rigs and…



DIY Superresolution, part III

Enrico Stefani’s lab at UCLA has a couple of crack engineers on board, Yong Wu and Pedro Felipe Gardeazábal Rodríguez. These two fellows built a custom STED system and have documented the setup on…



DIY Superresolution, part II

This paper (York et al. 2012) came out earlier this year, but I thought it’s worth highlighting here, give the subject matter.

We present a hybrid technique, multifocal SIM (MSIM), that combines the resolution-doubling…



DIY Superresolution

Quickpalm is an open source software package for DIY PALM and STORM implementations. They’re responsive on their discussion forum and have a wiki with a decent amount of technical information.



Whole embryo imaging

Drosophila embryos are a fraction of a square mm, and go from fertilization to hatching (as a larva) in about 22 hrs. So it’s possible to image individual embryos in their…



Z-Projections

To project 3D image stacks to a 2D image, several different algortithms can be used. Maximum projections and averages are the most commonly used. Neither…