A custom two photon scope on a wall
Posted in Hardware
![The figure shows the laser beam [in red, coming from the bottom right side of the panel (1)] that is first scanned by two galvanometric mirrors (2), then expanded by a telescope (3), and finally focused by the objective (4) onto the specimen (5). The emitted light (yellow) is separated from the exciting beam by a first dichroic mirror (6) and then split by a second dichroic mirror (7) in the red and green components. Two photomultipliers detect the split fluorescence emissions (8a,b).](http://labrigger.com/blog/wp-content/uploads/2012/09/wall.jpg)
This isn’t an overhead view of the table, they just built the whole thing on a vertically-mounted breadboard. Cool. (link)
![The figure shows the laser beam [in red, coming from the bottom right side of the panel (1)] that is first scanned by two galvanometric mirrors (2), then expanded by a telescope (3), and finally focused by the objective (4) onto the specimen (5). The emitted light (yellow) is separated from the exciting beam by a first dichroic mirror (6) and then split by a second dichroic mirror (7) in the red and green components. Two photomultipliers detect the split fluorescence emissions (8a,b).](http://labrigger.com/blog/wp-content/uploads/2012/09/wall.jpg)
This isn’t an overhead view of the table, they just built the whole thing on a vertically-mounted breadboard. Cool. (link)
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I’m surprised that this is not a much more commonplace practice in the space conscious labs in Europe. I’ve seen the two-tier setups with some launch optics on a second-level pillar-mounted breadboard more often, but it seems as if the vertical option might make components easier to reach for adjustment?
I hope you don’t mind me mentioning, but Micro Control Instruments now supply good value breadboards to European labs. Check out http://www.mci-neuroscience.com/product/optical-breadboards/
http://labrigger.com/blog/2012/02/29/tsiens-voltagefluors/
Does anyone know of a recent publication reviewing the latest developments of VF and other voltage sensitive dyes? And do current sCMOS cameras have the required characteristics to reliably record fast (1ms) events in neurons?
I’m surprised that this is not a much more commonplace practice in the space conscious labs in Europe. I’ve seen the two-tier setups with some launch optics on a second-level pillar-mounted breadboard more often, but it seems as if the vertical option might make components easier to reach for adjustment?
I hope you don’t mind me mentioning, but Micro Control Instruments now supply good value breadboards to European labs. Check out http://www.mci-neuroscience.com/product/optical-breadboards/