Tag: references

Detailed protocol for IV viral injections to drive systemic transgene expression

CRISPR/Cas9 technology has greatly accelerated the development of genetically engineered mice, but it’s still a big investment of time before the mice are ready. For quicker turnaround, there are other options (and speed isn’t the only reason…

Series resistance in patch clamp experiments

For teaching electrophysiology, there’s still a lack of comprehensive references. In particular, it can be difficult to impart to students an intuitive feel for the quality or fidelity of electrophysiological recordings. How close to the truth are those…

Tons of 2p spectra – twophotondyes.com


Daniel Fiole is curating a nice resource for 2p cross sections: twophotondyes.com

There’s a lot here. It’s not just dyes, he has links for fluorescent proteins as well, and there’s 3p data

iPad/iPhone/iOS app RayLab for optical design

RayLab is an iOS app (iPhone/iPad) for optics analysis. It has some nice features– more than I expected. It’s a nice piece of work! For many practical applications it cannot replace conventional optic design software (e.g., Zemax/OSLO/CodeV). That…

Enhancer trap mice from Brandeis

There’s a project in Sacha Nelson’s lab at Brandeis to generate and characterize enhancer trap mice for studying neural circuitry. They have a nice online searchable database of the lines they’ve generated, complete with histological…

New category of posts: papers

In the past, Labrigger has only rarely posted about relevant papers. That ends today. Now there’s a new post category for papers, and it’ll be used to highlight publications that are of potential interest to you, the community.

Lens designs

In the talk I gave at the Short Course at SfN 2014, I briefly discussed the process of optical system design. A common strategy is to start with a published design, or at least a general…

Photonics Handbook online reading

When you run out of catalogs to read, Photonics has some nice short articles. It’s all pretty basic, not too complicated. Good for training (e.g., Optical design software, Fiber lasers)

Practical guide to optical alignment

This is a nice, quick, and practical introduction to optical alignment. A good place to start for training. By Rainer Heintzmann.

Online app for comparing fluorescent proteins

George McNamara recently posted a comment on spectra, which referenced this online app which is handy.

A Canadian open source two-photon microscope system

This open source two-photon microscope system is adaptable for both slice (with substage detection) and in vivo experiments, and is built with largely COTS parts. The paper is a very nice resource.

See also, designs shared…

Notes from Austin Blanco

Our friend Christian Wilms tipped us to Austin Blanco’s blog, which has some posts you all might be interested in:

Characterizing unknown optical components
A few notes on Arduinos, their timers, and using them with rotary…

Abbe Diffraction setup

Kurt Thorn shared the parts list for his Abbe diffraction setup. He describes how to use it in his blog post, but you can also check out his talk.

Measuring the junction potential

How to measure the liquid junction potential

This is an old blog post, but the comments section is still somewhat active. Handy reference/training material.

Software Carpentry teaching programming and data management to scientists

Software Carpentry teaches scientists how to program and use open source tools to manage their data and make their life easier. They’re focussed specifically on software tools, rather than types of analysis, and so what they…