Monthly archives: November, 2015

Open source intrinsic imaging

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Leonardo Lupori and Raffaele Mazziotti are two fellows in lab of the excellent Tommaso Pizzorusso. They have developed an intrinsic signal optical imaging rig and are sharing all of the materials. Here’s their web site with…



Upconversion: NIR in, vis out

Compared to visible (vis) light, near infrared (NIR) wavelength scatters less and is less absorbed in brain tissue. If your fluorescent target absorbs vis light, then one way to use NIR is to flood the area with…



Coolant lines for lasers

Many lasers have baseplates with channels through which chilled water is pumped to dissipate heat and stabilize the temperature of the system. I haven’t had much trouble with the chillers and baseplates of Newport/Spectra-Physics systems. However,…



Intersectional strategies: CRE-DOG

Intersectional strategies are rapidly growing in sophistication. Unfortunately, it still takes time to generate mice and make crosses as needed, so short cuts are handy.

The CRE-DOG technique, from Connie Cepko’s lab, induces expression in cells that…



Imaging Course at Max Planck Florida

The Max Planck Florida Institute for Neuroscience is holding an imaging course Jan 11-20, 2016.
They have a really excellent line up of lecturers. A range of topics will be covered: two-photon imaging, FLIM, FRET, endoscopy, uncaging, optogenetics, SPIM, image analysis, instrumentation (alignment, system design), …

Applications are due in a few days, Nov….



Cute trick for spinning down tubes

The web site is in Japanese, but the pictures and gif are self explanatory.