Nov012013November 1, 2013November 1, 20131Commentby L.

Two photon technical notes

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The figure shows the laser beam [in red, coming from the bottom right side of the panel (1)] that is first scanned by two galvanometric mirrors (2), then expanded by a telescope (3), and finally focused by the objective (4) onto the specimen (5). The emitted light (yellow) is separated from the exciting beam by a first dichroic mirror (6) and then split by a second dichroic mirror (7) in the red and green components. Two photomultipliers detect the split fluorescence emissions (8a,b).

Here’s a collection of Labrigger posts, technical notes and whatnot, on 2-photon imaging.
2010 – Building a 2-photon microscope
2011 – Building a 2-photon microscope, 2011 edition
2010 – PMTs for 2-photon imaging
2011 – Photon counting and Hybrid PMTs
2011 – More on photon counting
2010 – Lasers for 2-photon imaging

More on two-photon imaging on Labrigger

MATLAB code

2011 – MATLAB code for collection optics part 1
2011 – MATLAB code for collection optics part 2

Calculations

2010 – Measuring the gain of a 2-photon imaging system
2010 – Pulsed laser power
2010 – Watts per photon

Some scopes available

UCLA Trachtenberg Scope
Thorlabs B-scope
Scientifica

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