My friend Bruno has written some very nice software for Scientifica’s two photon microscope systems. It’s called SciScan.
It’s written in LabVIEW and runs both their conventional galvo and resonant systems. These…
The working distance is marked as 22 mm in this image, which is measured on-axis, from the center of the last lens. Practically, the mechanical working distance will be 20 mm.
We have designed a 20 mm working distance…
Daniel Fiole is curating a nice resource for 2p cross sections: twophotondyes.com
There’s a lot here. It’s not just dyes, he has links for fluorescent proteins as well, and there’s 3p…
Olympus released a suite of new objectives this year, and one of them is this 10x/0.6 NA. This is probably the longest focal length (180/10 = 18 mm) objective you can get and use with a…
This open source two-photon microscope system is adaptable for both slice (with substage detection) and in vivo experiments, and is built with largely COTS parts. The paper is a very nice resource.
See also, designs…
Adrian Negrean, in Huibert Mansvelder’s lab, has been designing and building custom scan optics for two-photon imaging and earlier this year published his work (paper). Labrigger wants to emphasize how nice this paper is. It’s an…
Dario Ringach has written some nice software for the Trachtenberg scope mentioned before on Labrigger. They also have put together their own Cypress PSoC-based hardware box to control several parts of the system.
He set up…
Here’s an update on the scope from Joshua Trachtenberg’s team. If you want one, contact them at: info.neurolabware@gmail.com
This is another moving objective microscope, like the Sutter (Denk/MOM) scope, and the Thorlabs scope. Here’s a couple…
Perhaps you’re familiar with the various AOD and piezo-based methods for two-photon imaging in 3D quickly. Here’s another way (Botcherby et al. 2012, PNAS, open access)
The used all galvos, rather than AODs or piezo…
It’s difficult to track down 2p absorption cross sections for dyes and proteins. Doing it right requires (ideally) fluorescence…
We just updated the preprint for the Cousa objective. It’s a big update.
Back in November 2020, we announced a design for a 20 mm working distance objective optimized for…
Andrew Lim wrote in to discuss strategies on dealing with ripple noise in 2-photon imaging systems, particularly when using resonant scanners. He writes:
This isn’t so much a tip as a problem with resonant two-photon scopes that…
The preprint for the wide field of view 2-photon system we built has been updated. Check it out at bioRxiv.org.
Preprint servers are a great way to share work-in-progress. ArXiv and bioRxiv…
