SIMA – Image analysis from the Losonczy lab

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(This post by the SIMA Team.)

The SIMA (Sequential IMage Analysis) package facilitates analysis of time-series imaging data arising from fluorescence microscopy. The functionality of this package includes:

– correction of motion artifacts
– segmentation of imaging fields into regions of interest (ROIs)
– extraction of dynamic signals from ROIs

The included ROI Buddy software provides a graphical user interface (GUI) supporting the following functionality:

– editing of ROIs resulting from automated segmentation
– registration of ROIs across separate imaging sessions

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