Cough syrup

My MD friends tell me there’s no effective cough medication on the market. There are OTC and prescription medicines, but none of them have been proven to really work. That doesn’t mean that they won’t give you some level of subjective relief, but they’re not like asprin or pseudoephedrine (e.g., regular Sudafed)*.

We used to…



Imaging deeper with 3p

One of the factors that limits the depth at which one can image brain tissue in vivo with two-photon imaging is out-of-plane fluorescence. Although the excitation falls off as the square of the distance from the focal…



Converting miles to km with Fibonacci

Previously on Labrigger: Street fighting mathematics

And on that topic, and this is something the runners among us might appreciate, the Fibonacci sequence can be used to easily convert miles to kms.

Take two consecutive Fibonacci numbers,…



Going back to Cali

No. I don’t think so.
Anyways, CaliCode is a resource for population calcium imaging. It covers topics from inferring action potential trains from imaging data (hat tip to JV), to image processing and topical…



Methods of Mayhem

I’ve seen this line in a methods section before:
“Experiments were carried out at room temperature (19-29 degrees C).”

Which means: “Our lab is in a building with no A/C and poor heating.”

For…



More references

Wolfgang Becker just gave a nice talk at the Berkeley Advanced Imaging Methods meeting.

I should add the Becker-Hinkl literature to the list of online references.

Previously
Catalogs as textbooks
Troubleshooting fluorescence microscopy



PubPeer Anonymous

You can now post comments on PubPeer completely anonymously. Not even a login is required.

PubPeer has always allowed anonymous comments, but it used to be that you had to log in, so the…



Markram’s Millions

Henry Markram got his wish. He was just awarded 500,000,000 Euros to build a computer model of the brain. In NIH terms, that’s over…



Electrophysiology software

Unenthusiastic about spending thousands of dollars on electrophysiology software like pCLAMP? Try the free and open source Strathclyde Electrophysiology Software. It’s a suite of programs for recording and analyzing signals from intracellular electrophysiology…



Get the computation done

Programming that happens in labs is typically rushed and sloppy. The goal is to get the job done right, and, almost…



Make MATLAB text you

Patrick Mineault covered this back in November in his always interesting xcorr…



Online course materials

There are a lot of online, open course materials, e.g. MIT’s OpenCourseWare



Photoswitchable GECI

Many red fluorescent proteins go through a green fluorescent stage prior to becoming fully folded into their mature red fluorescent state. In fact, some proteins can be photoswitched in and/or out of their mature…



EMCCD Specs

Editorial preface: Labrigger takes submissions from anyone. We post them if they’re potentially useful. In this case, we received some material from a commercial source. Labrigger is not endorsing their products. But we recognize…



PubPeer is taking off

PubPeer, a site for anonymous (or non-anonymous), post-publication peer review is taking off nicely. Traffic is increasing to the site and there are a lot of interesting comments, including both detailed commentary