The lasers used in multiphoton imaging deliver their photons in pulses. Many commonly used systems pulse at 80 MHz. However, there are good reasons to try different frequencies.
In 2007, Donnert, Eggeling, and Hell published a Nature Methods paper where they used low frequency pulses to get more fluorescence…
Lasers for 2-photon microscopy are special in that they deliver all of their photons in a short period of time. Take a standard Ti:Sapph with an 80 MHz pulse rate and 100 fs pulses. If each…
Someone asked me recently how peak power related to average power for pulsed lasers. You can get fancy with this calculation, but people rarely do. 99% of the time, people assume…
Fiber optics must be terminated properly in order to permit high transmission. If you’re making your own, get a proper fiber cleaver and a set of polishing sheets. Here are a few…
Here are some 2p cross sections for Alexa dyes from Invitrogen. Dye manufacturers are getting better about sharing 2p cross sections, but they still rely on out side people to do the measurements. In this case, Warren Zipfel from Cornell.
Speaking of Cornell,…
It’s difficult to track down 2p absorption cross sections for dyes and proteins. Doing it right requires (ideally) fluorescence…
Coherent just sold its 1000th Chameleon system. That’s roughly how many of the similarly-priced Lamborghini Countach LP500s were sold. That was in the 1980s. Coherent has been selling Chameleons since 2003,…
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