MATLAB code for designing collection optics for 2p scopes
Posted in Software
The Tsai book chapter from Ron Frostig’s CRC book is a great resource for building your own 2p microscope. In section…
White House budget proposal 2012
Posted in Uncategorized
Funding is tight all over. The question is only: How tight? The White House released its budget today. It’s just a proposal, and is subject…
Repurposing audio recording products
Posted in Hardware
Scientific research equipment is expensive. Partly because the markets are often small, so product development budgets and manufacturing set up costs have to be split over a comparatively small number of units. In addition, the customers are typically more concerned about reliability and service, rather than price.
However, there are other markets where these issues are…
Reality check for the Octavius-2P laser
Posted in Hardware
Lasers for 2-photon microscopy are special in that they deliver all of their photons in a short period of time. Take a standard Ti:Sapph with an 80 MHz pulse rate and 100 fs pulses….
In over your head
Posted in Uncategorized
My Ph.D. program had a more traditional, comprehensive written qualifying exam following the coursework. However, many of my contemporaries in different programs had the new…
Fluorescence retrofit
Posted in Hardware
This is a cute little product that lets you retrofit a standard microscope for fluorescence. It contains an LED light source, dichroic, and filters. It may be clumsy to have this big blue cannon…
Some bits from others
Posted in Uncategorized
Similar to the last post, here are some more choice bits from other people:
New vocab
Helpful Vocabulary for Journal Reviewers
The post offers some…
Tips for giving talks
Posted in Tips
This is just a quick post to point people over to Matt Might’s excellent post full of tips on how to give a good scientific talk. I second his endorsements of Keynote and…
Hopes for the New Year
Posted in Tips
Here’s the news Labrigger hopes to see in 2011.
New developments with optical indicators
A new, super bright fluorescent protein that blows the doors off of GFP-based proteins.
Bright, far-red dyes with huge 2p cross-sections.
Higher S:N genetically-encoded…
Pubmed limbo
Posted in Uncategorized
In my experience, PubMed works beautifully the vast majority of the time. It does an excellent job parsing search terms and they’re always adding new features (e.g., you can search using full…
Open source orbital shaker
Posted in Hardware
This is a nicely done (and award-winning) orbital shaker. Orbital shakers can often cost on the order of $1500. This one can be custom built for about $150, with additional units costing about $60 (a single controller and power…
Watts per photon
Posted in Uncategorized
Fun conversion of the day: photon count to watts. This is high school physics, but it’s a good reminder of…
Shot noise-limited imaging
Posted in Uncategorized
What is the advantage of imaging fast in population calcium imaging? Here’s a quick qualitative comparison. The prep was mouse visual…
Future Publishing
Posted in Tips
We used to just tell stories. Then we had monks copy manuscripts by hand. Later, movable type made printing easy. That brings us up to about…
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