3D Printed Equipment from the DeRisi Lab
Posted in Hardware
The DiRisi Lab at UCSF shares their STL files for 3D printing some equipment. There’s a pipette holder, several gel combs, an objective case, and more. You can print your own with your…
Single cell electroporation workshop
Posted in Tips
Michael Hausser’s lab is having a single cell electroporation workshop in London November 1-2. (link)
The application deadline is Thursday, September 30, 2010.
…
DIY open access
Posted in Uncategorized
Even if you publish in a “closed” journal, you are typically allowed to post the PDF of your article on your…
Open Science Summit videos
Posted in Uncategorized
There are videos from the talks at the Open Science Summit online now. The topics include the OpenPCR and Otyp projects, open source drug discovery,…
Building a 2-photon microscope
Posted in Hardware
Less than a year ago, the patent on 2-photon microscopy ran expired (Nov 14, 2009 pdf). So now anyone can sell a kit or system for two photon microscopy without having to pay a licensing fee.
If you don’t want to start from scratch, there are a lot of options.
Prairie Technologies
Open science
Posted in Uncategorized
“Open” is a nice sounding word, not unlike “free”, that is used rather liberally whenever some bit of intellectual property is shared with the public. However, it can mean very different things…
Multichannel recording systems
Posted in Hardware
This is just a quick list of manufacturers/vendors of multielectrode recording hardware, and their roots. Unsurprisingly, they were almost all started by neurophysiologists. It’s interesting how diverse they are, they each seem to…
Lasers for multiphoton microscopy
Posted in Hardware
Coherent just sold its 1000th Chameleon system. That’s roughly how many of the similarly-priced Lamborghini Countach LP500s were sold. That was in the 1980s. Coherent has been selling Chameleons…
Outfitting a lab on the cheap
A friend of mine does a lot of molecular biology and keeps an incubator at home so she can run a reaction and stop it some time at night without having to make…
Remote desktop software
Posted in Software
From time to time, you might catch me on my yacht, running an imaging experiment by remote desktopping into my rig from my laptop. I would use my iPhone, but I can’t fit all the windows for ScanImage onto the screen, even…
TIFFs in MATLAB
Posted in Software
MATLAB uses the open source library libtiff to write TIFF files, or they did the last time I asked. I work with multipage TIFFs a lot, and I find MATLAB is not…
Crowdsourcing your work
Posted in Uncategorized
Ebird is a website where bird watchers (or “birders”, in the parlance of the field) upload their observations. This project, by Cornell’s Lab of Ornithology and the National Audubon Society, has been…
Soft raw materials
Posted in Tips
A UK postdoc came to our lab in the US and was surprised that we didn’t have any Blu-Tack in the lab. His old lab wasn’t the only UK lab that goes…
EM and fluorescence analysis in the same preparation
Posted in Hardware
Several labs have set up their own workflows for imaging the same tissue using both fluorescence and electron microscopy (EM). Zeiss just released a product, called Shuttle-and-Find, to streamline this. It’s pretty simple,…
Structured illumination
Structured illumination can be used to increase the resolution of wide field fluorescence imaging. The idea is best motivated by thinking about Moire fringes. In panel (a) below, if you stand away from…
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