Post by Darcy Peterka (Columbia University/Yuste lab):
We do a bunch of multiple imaging/uncaging experiments with 2+ lasers, and got tired of constantly taking apart the camera/laser ports to switch lenses and dichroics. This…
Structured illumination can be used to increase the resolution of wide field fluorescence imaging. The idea is best motivated by thinking about Moire fringes. In panel (a) below, if you stand away from the screen…
Microscope objectives, in particular, the water-dipping variety so common in physiology and imaging labs, get dirty. Salts build up on them, traces of drugs stick to them, and biological membranes accumulate. It’s best to…
Look at this transmission spectrum from Semrock. It’s a nearly perfect step function. You want to know about the optical density outside of the transmission band? Over 7 outside of the 500-550nm band….
I recently covered some alternative optomechanics sources beyond the usual suspects like ThorLabs and Newport. Here are a couple of less well known optomechanics shops.
The unfortunately named Eksma Optics has a…
Low magnification, high numeric aperture (NA) microscope objectives are popular choices for multiphoton imaging. They offer excellent light collection properties, a wide field of view, and still permit high resolution imaging. However, they are…
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