Test slides
Let me tell you a little story.
The place is the CONFOCALMICROSCOPY listserv.
The time is 1997.
A query asks how to mount fluorescent beads from Molecular Probes in order to image them…
Category: Tips
OpenOptogenetics
When Karl Deisseroth started publishing his work on Channelrhodopsin-2, he set up a website to share the resources, including plasmid information, protocols for expression systems, and hardware details. His site, optogenetics.org, is…
Tips for giving talks
Posted in Tips
This is just a quick post to point people over to Matt Might’s excellent post full of tips on how to give a good scientific talk. I second his endorsements of Keynote and…
Hopes for the New Year
Posted in Tips
Here’s the news Labrigger hopes to see in 2011.
New developments with optical indicators
A new, super bright fluorescent protein that blows the doors off of GFP-based proteins.
Bright, far-red dyes with huge 2p cross-sections.
Higher S:N genetically-encoded…
Future Publishing
Posted in Tips
We used to just tell stories. Then we had monks copy manuscripts by hand. Later, movable type made printing easy. That brings us up to about…
Pulsed laser power
Someone asked me recently how peak power related to average power for pulsed lasers. You can get fancy with this calculation, but people rarely do. 99% of the time,…
Labrigger Xmas Gift List 2010
Here’s the 2010 Labrigger Xmas Gift List.
Care to add anything?
Beam profiler
Measure the divergence of your laser beam, its stability, uniformity– all kinds of good stuff. These are useful metrics for diagnosing problems with the laser and really nailing the…
Ponoko starts 3D printing
Posted in Tips
Ponoko is a laser cutting service that recently started offering 3D printing. Their 3D services is similar to what Shapeways offers. It’s nice to see a pair of…
Raw materials
Posted in Tips
If you need a chunk of metal for a custom piece for your rig, what are the options? The best way to go is to buy a part that is close to what you…
Fiber optic termination
Posted in Tips
Fiber optics must be terminated properly in order to permit high transmission. If you’re making your own, get a proper fiber cleaver and a set of polishing sheets. Here are…
More 2p Cross Sections
Posted in Tips
Here are some 2p cross sections for Alexa dyes from Invitrogen. Dye manufacturers are getting better about sharing 2p cross sections, but they still rely on out side people to do the measurements. In this case, Warren Zipfel from Cornell.
Speaking of…
Two-Photon Cross Sections
Posted in Tips
It’s difficult to track down 2p absorption cross sections for dyes and proteins. Doing it right requires…
CAD-based optomechanics mock-ups
I use SolidWorks to design custom parts for my rigs. Many times, these parts mate with existing Thorlabs parts. Fortunately, Thorlabs offers SolidWorks files that can be directly…
RSS feeds for any web page
Posted in Tips
I follow quite a number of RSS feeds, but there are some news sites that don’t have them. In order to keep up to date, use Page2RSS.
For example, if Nature Neuroscience didn’t have an RSS feed for their Advanced Online…
Feeds and Forum
Recent comments
- Consensus reality is a public good – Labrigger on Research Budgets in Perspective 2020
- L. on Snappy looking calcium imaging videos
- Ian on Two-Photon Cross Sections
- Typst template for preprints – Labrigger on Switching to Typst
- Typst template for preprints – Labrigger on 10 quick start items for Overleaf to Typst
- Problems with system comparisons – Labrigger on Axial resolution vs numerical aperture
- Rigor for everyone – Labrigger on Accountability is a way to determine truth
- Rigor for everyone – Labrigger on Entire fields on poor foundations
- L. on Snappy looking calcium imaging videos
- Peter Rupprecht on Snappy looking calcium imaging videos
Tag cloud
3d printing analysis arduino behavior calcium imaging collaboration computing construction course data acquisition dissemination electronics electrophysiology equipment fluorescence funding gadgets imaging jobs labview laser laser cutting machining manipulators materials MATLAB meeting microscopy openness optics osx pcr processing python references robotics two-photon video visual stimuli water jet cutting- June 2026
- March 2026
- February 2026
- January 2026
- December 2025
- November 2025
- September 2025
- August 2025
- July 2025
- June 2025
- May 2025
- April 2025
- February 2025
- January 2025
- November 2024
- June 2024
- May 2024
- April 2024
- March 2024
- February 2024
- January 2024
- December 2023
- November 2023
- October 2023
- September 2023
- August 2023
- July 2023
- June 2023
- May 2023
- March 2023
- January 2023
- November 2022
- October 2022
- September 2022
- July 2022
- June 2022
- March 2022
- September 2021
- November 2020
- July 2020
- June 2020
- May 2020
- February 2020
- November 2019
- October 2019
- June 2019
- December 2018
- November 2018
- October 2018
- July 2018
- June 2018
- May 2018
- April 2018
- March 2018
- February 2018
- January 2018
- December 2017
- November 2017
- October 2017
- September 2017
- August 2017
- July 2017
- June 2017
- May 2017
- April 2017
- March 2017
- February 2017
- November 2016
- September 2016
- August 2016
- July 2016
- May 2016
- April 2016
- March 2016
- February 2016
- January 2016
- December 2015
- November 2015
- September 2015
- August 2015
- July 2015
- May 2015
- April 2015
- March 2015
- February 2015
- January 2015
- December 2014
- November 2014
- October 2014
- September 2014
- August 2014
- July 2014
- June 2014
- May 2014
- April 2014
- March 2014
- February 2014
- January 2014
- December 2013
- November 2013
- October 2013
- September 2013
- August 2013
- July 2013
- June 2013
- May 2013
- April 2013
- March 2013
- February 2013
- January 2013
- December 2012
- November 2012
- October 2012
- September 2012
- August 2012
- July 2012
- June 2012
- May 2012
- April 2012
- March 2012
- February 2012
- January 2012
- December 2011
- November 2011
- October 2011
- September 2011
- August 2011
- July 2011
- June 2011
- May 2011
- April 2011
- March 2011
- February 2011
- January 2011
- December 2010
- November 2010
- October 2010
- September 2010
- August 2010
- July 2010
- June 2010