Collection optics: How big is big enough?
Posted in Hardware
To advance deep imaging in multiphoton microscopy, it is essential to attempt to collect each photon generated in the…
Search Results for "two photon"
Headplate designs
Posted in Hardware
Multiphoton imaging with micron resolution in living animals can be challenging…
Patents and academic research
Posted in Uncategorized
This is about the research exemption for patents. Early in my training, I was taught that academic researchers didn’t need to worry about patents, because they could build whatever they wanted for research purposes without licensing…
Postdoc position at Imperial College London – optics, holography, neuroscience, optogenetics
Posted in Tips
Post by Amanda Foust
Applications are invited for the above post to work with Dr. Amanda Foust (Bioengineering department) and Prof. Pier Luigi Dragotti (Electrical and Electronic Engineering department) on a BBSRC funded project…
PMT power supply
I was cleaning house a bit, and among my old files I found this, which might be worth sharing. Years ago I made a centralized power supply for a custom 2-photon imaging system I built. There were…
Upconversion: NIR in, vis out
Posted in Tips
Compared to visible (vis) light, near infrared (NIR) wavelength scatters less and is less absorbed in brain tissue. If your fluorescent target absorbs vis light, then one way to use NIR is to flood the area…
Imaging Course at Max Planck Florida
Posted in Uncategorized
The Max Planck Florida Institute for Neuroscience is holding an imaging course Jan 11-20, 2016.
They have a really excellent line up of lecturers. A range of topics will be covered: two-photon imaging, FLIM, FRET, endoscopy, uncaging, optogenetics, SPIM, image analysis, instrumentation (alignment, system design), …
Applications are due in a few days, Nov….
Voice control
Voice control has just recently become interesting to me. Ikuko bought me an Amazon Echo and I’m surprised at how useful I find it. I’m also surprised at how well it works– it can decipher…
FocusStack and StimServer for MATLAB
Posted in Software
Dylan Muir and Bjorn Kampa created some MATLAB code for two-photon calcium imaging experiments. First up is FocusStack, which provides a suite of analysis tools. Next up is StimServer, which coordinates visual stimulus generation and presentation….
Reminder: SfN Short Course
Posted in Tips
If you like Labrigger, you should register for this Short Course at the SfN meeting.
Short Course #1: Advances in Multineuronal Monitoring of Brain Activity
Spencer Smith will be talking about the work …
Postdoc wanted for the SLAB
Posted in Uncategorized
SLAB is looking for a postdoc. This postdoc’s initial project would involve mouse behavior and in vivo two-photon calcium imaging. Prior neurophysiology work, particularly with in vivo experiments, is highly recommended. Competitive applicants will…
Visual stimulation and intrinsic imaging code
Posted in Software
Ian Nauhaus, whom UT Austin recently nabbed, is sharing his code for intrinsic imaging and visual stimulation.
The visual stimulation code is based off of Psychophysics Toolbox, which is already in use by…
Commoditizing population calcium imaging analysis
Posted in Software
More open source software to check out.
Two-Photon Processor and SeNeCA – A freely available software package…
Imaging deeper with 3p
Posted in Uncategorized
One of the factors that limits the depth at which one can image brain tissue in vivo with two-photon imaging is out-of-plane fluorescence. Although the excitation falls off as the square of the distance from the…
EMCCD Specs
Posted in Hardware
Editorial preface: Labrigger takes submissions from anyone. We post them if they’re potentially useful. In this case, we received some material from a commercial source. Labrigger is not endorsing their products. But we recognize that…