Interview with the developers of HelioScan
Last Monday, Labrigger covered HelioScan, a LabVIEW-based, two-photon laser scanning microscopy software suite.
Marcel van ‘t Hoff (left) and…
Search Results for "two photon"
Axial resolution and numerical aperture, part II
Posted in Tips
To recap the previous post on axial resolution and numerical aperture in two-photon microscopy:
For excitation deep in scattering tissue, higher NA can actually be detrimental because the light cone at…
Spectra viewing app for iOS
Posted in Software
Zeiss released an iOS app for viewing spectra.
No Android version yet, but Johannes Amon said:
of course I can’t tell you any specifics but at the moment we are evaluating a native…
Optimizing pulse frequency for 2p imaging
Posted in Uncategorized
The lasers used in multiphoton imaging deliver their photons in pulses. Many commonly used systems pulse at 80 MHz. However, there are good reasons to try different frequencies.
In 2007, Donnert, Eggeling, and Hell published a Nature Methods paper where they used low frequency pulses to get more…
Building a 2p scope, 2011 edition
Posted in Hardware
What’s new this year?
The original post on building a 2p scope still gets a lot of hits. Let’s revisit the topic.
Scientifica
Scientifica has been working hard on developing a kit for multiphoton imaging. It’s all based off of their minimalist, yet versatile SliceScope platform.
I’ve had the opportunity to check out their collection module and…
More Spectra, Please
Posted in Tips
George McNamara is planning on updating the massive PubSpectra (previously mentioned here).
He’s currently soliciting submissions. Do you have any to contribute?
(link)
…
2pLSM: When will scientists stop making their own?
Posted in Hardware
In the early days of patch clamp electrophysiology, everyone made their own patch clamp amplifiers because there were none commercially available. I was lucky enough to be educated by scientists of such lineage, and…
2p cross sections for fluorescent proteins
Posted in Tips
On the off chance that you missed it, Drobizhev et al. published a bunch of 2p cross sections for fluorescent proteins.
Earlier:
2p cross sections
More 2p…
Apps
Posted in Software
You probably caught the article about the group who runs their laser-trapping experiments using an app they wrote for iOS on the iPad. This sparked a commentary about apps in science. What about some other apps for handheld and tablet computing?
Reference organization
In general, when it comes…
Thorlabs scope building blocks
Posted in Hardware
At the SfN meeting in San Diego this year, I met Jeff Brooker from Thorlabs. Jeff and his team of about 17 people have a history of working together in industry on photonics. They have…
More 2p Cross Sections
Posted in Tips
Here are some 2p cross sections for Alexa dyes from Invitrogen. Dye manufacturers are getting better about sharing 2p cross sections, but they still rely on out side people to do the measurements. In this case, Warren Zipfel from Cornell.
Speaking of…
Optomechanics for a custom 2p uncaging rig
Posted in Hardware
Post by Darcy Peterka (Columbia University/Yuste lab):
We do a bunch of multiple imaging/uncaging experiments with 2+ lasers, and got tired of constantly taking apart the camera/laser ports to switch lenses and dichroics. This…
Measuring the gain of your imaging system
Posted in Hardware
The images obtained from a 2-photon laser scanning microscope, or a CCD-based system, consist of an array of pixel values. For 8-bit…
Intrinsic imaging tips
Posted in Tips
Intrinsic signal optical imaging is a functional imaging modality where the reflectance of red light indicates active portions of cortex. It is used for many applications, including imaging individual barrels in rodent somatosensory…
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