Democratized state-of-the-art neuroscience

Andre and colleagues have a preprint up on their project generating inexpensive instrumentation for imaging and optogenetics experiments. The 100 Euro Lab: A 3-D Printable Open Source Platform For Fluorescence Microscopy, Optogenetics…



March for Science – Apr 22

OSA – Optical Society of America
SfN – Society for Neuroscience
March for Science



Nature is diluting their brand

From MIT.
Hat tip to J.S.

More print-outs:
Flyer in the Physics building
Kawaii psychophysics



Actually useful comments on scientific blogging

I came across Rachel Thomas’ comments on blogging in the context of machine learning, and they’re good. I’ve been telling people things like this for awhile now and Rachel distills them better than I do. I’ll…



PMT power supply

I was cleaning house a bit, and among my old files I found this, which might be worth sharing. Years ago I made a centralized power supply for a custom 2-photon imaging system I built. There were two…



Driving a green laser pointer with MicroManager

Reminder: green laser pointers have a ton of IR in them. It needs to be filtered out if you don’t want it. See this old (2011!) Labrigger post.

I.e., Paul and Kurt are right (see…



Pixy computer vision for tracking mouse behavior

Pixy is an open source computer vision system. Mostafa Nashaat, Robert Sachdev, and colleagues including Matthew Larkum have developed software for use with the Pixy, that can be used to track mouse behavior, including free movement around an…



Analysis algorithms: performance quantification and ground truth

We recently tweeted about a preprint from Eftychios A Pnevmatikakis and Andrea Giovannucci (code). The preprint is on motion correction for calcium imaging data. It is a nice quick read and discusses earlier work in the…



Intan CLAMP Patch Clamp Amplifier Systems

Multiple people have asked for a discussion of Intan CLAMP patch clamp amplifiers. For those who are unfamiliar with them, they’re miniaturized patch clamp amplifiers. What looks like a headstage, is actually an…



The breadboard you didn’t know you wanted

It’s a microcontroller built into a breadboard. Actually, TWO microcontrollers. Both are Arduino-compatible. ATmega16U2 and ATmega328P.
It comes in both black, white, and pink because style matters.

P.S. The bottom side is filled with…



Series resistance in patch clamp experiments

For teaching electrophysiology, there’s still a lack of comprehensive references. In particular, it can be difficult to impart to students an intuitive feel for the quality or fidelity of electrophysiological recordings. How close to the truth are…



Imaging course at Max Planck Florida

Max Planck Florida is running their imaging course again and there’s still time to apply. They’ve got great faculty including Na Ji, Ryohei Yasuda, Yi Zuo, Chris Xu, Jeff Lichtman, Naomi Kamasawa, and more!



Pre-preprint: blogging a project

SLAB is trying something new with one project in the lab. Prior to drafting a preprint, we’re blogging the project and sharing the results and analysis. We invite anyone to comment on the…



Allen Institute’s Brain Observatory data set

The Allen Institute has released the first set of data from their Brain Observatory project. Many of you already know about this, but I wanted to post about it to encourage people to take a…



Tons of 2p spectra – twophotondyes.com

 

Daniel Fiole is curating a nice resource for 2p cross sections: twophotondyes.com

There’s a lot here. It’s not just dyes, he has links for fluorescent proteins as well, and there’s 3p…