GIMP 2.8
Posted in Software
GIMP is an open source image editing program. You can use it for a lot of the same things Adobe Photoshop is used for.
They just released version 2.8 (release notes).
In addition to GIMP,…
Tag: imaging
Light field imaging
Posted in Hardware
Light field imaging is a very interesting approach to imaging. The idea is to use an array of microlenses in order to capture light coming from many different angles. By storing this data, it is…
Axial resolution and numerical aperture, part II
Posted in Tips
To recap the previous post on axial resolution and numerical aperture in two-photon microscopy:
For excitation deep in scattering tissue, higher NA can actually be detrimental because the light cone at…
Axial resolution vs numerical aperture
Posted in Tips
Recently, microscope manufacturers have been releasing ever higher NA objectives for multiphoton imaging. Although higher NA objectives should give better axial resolution, they might not be ideal for imaging deep into the…
3D Printed iPhone-to-Eyepiece adapters
Posted in Hardware
Thanks go to commenter ybot for this one. They pointed out this handy iPhone-to-Microscope mount iPhone case.
From the pictures, it looks like the prototype was printed on a Makerbot, which isn’t terribly high…
Tsien’s VoltageFluors
Posted in Tips
Roger Tsien’s lab recently published the new generation voltage sensitive dye they were presenting at SfN: VoltageFluors. As often when then Tsien Lab takes on a new field, they start by taking a completely…
Intrinsic imaging on the cheap
Posted in Hardware
Intrinsic signal optical imaging is a functional imaging modality where the reflectance of red light indicates active portions of cortex. It is used for many applications, including imaging individual barrels in rodent somatosensory cortex, maps in visual cortex, and the tonotopic organization in auditory cortex.
Here is a prior Labrigger post with tips for intrinsic…
Clearing tissue for imaging: index matching is not new
Posted in Hardware
The main factor that limits how deep we can image into tissue is the scattering of light. Multiphoton imaging partially mitigates the problem by using infrared light, which scatter less, and by using an excitation…
MATLAB command locateVal
Posted in Software
In two previous posts I shared some MATLAB code to help design collection optics in 2p scopes.
Collection optics for 2p scopes, post 1
Collection optics for 2p scopes, post 2
It was just brought to my attention that I didn’t include the command locateVal in the code I posted. It’s a very…
Use ScanImage? Support it. It’s free.
Posted in Software
ScanImage is an excellent software package for controlling 2p scopes. It’s free and open source. It’s been actively developed and released to…
Optimizing pulse frequency for 2p imaging
Posted in Uncategorized
The lasers used in multiphoton imaging deliver their photons in pulses. Many commonly used systems pulse at 80 MHz. However, there are good reasons to try different frequencies.
In 2007, Donnert, Eggeling, and Hell published a Nature Methods paper where they used low frequency pulses to get more…
An Arch-based voltage sensor
Posted in Uncategorized
The newest voltage sensor is from Adam Cohen’s lab and based on a microbial rhodopsin, a class of light sensitive proteins already being put to use in optogenetics.
In this case, they’ve take…
Thorlabs’ B-scope
Posted in Hardware
Thorlabs’ scope pieces and kits have been mentioned in these pages before. At SfN, they had their new B-scope on display. This is like the Sutter MOM and the UCLA…
TIRF teaching materials
Posted in Tips
Next time you’re explaining TIRF to someone, start off by showing them this piece of artwork. (Arndt von Scheidt, source)
…
Building a 2p scope, 2011 edition
Posted in Hardware
What’s new this year?
The original post on building a 2p scope still gets a lot of hits. Let’s revisit the topic.
Scientifica
Scientifica has been working hard on developing a kit for multiphoton imaging. It’s all based off of their minimalist, yet versatile SliceScope platform.
I’ve had the opportunity to check out their collection module and…
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